ANTIXX Fire Ant Bait contains spinosad, a naturally occurring soil organism. ANTIXX Fire Ant Bait is attractive and deadly to foraging red imported fire ants (Solenopsis invicta) and harvester ants and is readily taken back into the nest as food for the colony. The active ingredient in the bait is distributed throughout the colony, killing the ants, including the queen. Fire ants and harvester ants that consume the bait will appear sick and lethargic within 12 to 24 hours. Dead ants will frequently be seen near treated mounds 24 to 36 hours after application. The entire colony, including the queen, will usually be controlled within 3 to 14 days after treatment, depending upon environmental conditions. Control of extremely large colonies may take longer or may require reapplication. If reapplication is required, use specified mound or broadcast application rates at intervals of no less than 14 days until control is achieved. Control may be slower with broadcast applications than with individual mound treatments.